MACROMOLECULAR BIOCHEMISTRY
CHEMISTRY 333

Dr. Terry L. Helser, Associate Professor of Chemistry
227 Physical Sciences Building, Oneonta, NY 13820-4015
Phone: (607)436-3518 or Email to: HELSERTL@oneonta.edu. 
Study Guide - From GENES To CELLS, 1997, S. R. Bolsover, et al. (John Wiley & Sons)
Chapter 6: Patterns of Inheritance

You can go back to the Spring schedule, to the next extra credit puzzle or project, or leave me a message. Or skip to chapter 7.


 What was Gregor Mendel's theory of heredity?

How has the definition of the "gene" changed with time?

Who and what proved genes are matter and part of chromosomes?

Define phenotype, genotype, allele, dominant and recessive. Also, diploid, haploid, homozygous and heterozygous.

What two laws of heredity did Mendel propose? How do alleles of a single trait behave in a cross (that is, independent segregation)? How do alleles of different traits behave (that is, independent assortment)?

How is a pedigree used to show inheritance of human genes? Know specific examples like Huntington's disease, blood types, cystic fibrosis, etc..

What is incomplete dominance? How does it differ from codominance? How do these help to prove Mendel's particulate theory of heredity?

Can lethal alleles be dominant? What is the relevance of recessive lethals in humans?

Which parent gave you your mitochondrial genes? This is an example of ____________ _________.

Why are there different alleles? Define an auxotroph.



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Ch. 7: Mapping the Genes

What are sex-linked traits? Give an example from Drosophila and humans.

Which of Mendel's laws is, fortunately, only partly correct?

What is linkage? What is the significance of linkage maps? ...of linkage groups? How does a physical map relate?

What is a complementation test? What does it tell you about mutants?

What are introns and exons? How are they involved in evolution?
How are duplications and pseudogenes involved?

Why is one person a male and another female?



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Microbial Genetics
What advantages do microbes have over eukaryotes for genetic studies?

How do organisms grow? Plot the graph that describes cell/organism growth with time. Define and label the lag, log and stationary phases. What would you expect the last phase to be?

If you start with a culture of E. coli at 1x104 cells/mL, how many cells/mL will you have after 2 hours? This strain grows with a generation time of 30 minutes in your medium.

What are the two major reasons for cells to enter a stationary growth phase?

How were auxotrophs and prototrophs used to select for true recombinants in E. coli? How did Lederberg rule out revertants, cross-feeding and transformation?

How is conjugation used to map genes in E. coli? What did this show about the structure of the genome in  E. coli?

How does an episome differ from a plasmid? Which is the fertility factor, F, in E. coli?
What is the difference between an F-, F+, Hfr and an F' merodiploid?

How can you do a complementation test in E. coli?

What is the name given to bacterial viruses? How do you detect their presence?

What differs between a lytic life cycle with its eclipse period, and a lysogenic response with its prophage state.

Be able to define and understand the difference between conjugation, transduction, transformation and transfection.



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  Last Modified on 1/5/99