Bread & WineEnzyme Catalyzed Reactions

What follows is a list of dyes that can be used as substrates for plant peroxidases. The table contains ordering information from the 1998 catalogues of Sigma Chemical Co. and Research Organics, Inc. if available. You can also jump to other lab procedures in an appendix, the biochemistry lab schedule, the biochemistry page or the addresses at the bottom.
 
Acronym
F.W.
Name
Sigma
Cat #, p.
Cost/Amt
Res.Org
Cat #
Cost/Amt
Rxn. Product
Color
Wavelength
ABTS
548.7 dal
2,2'-Azino-bis(3-ethylbenzthiazoline-
6-Sulfonic Acid´2 NH4 salt
A 1888
p. 161
1 g/$22.10
5 g/$85.35
10 g/ $153.80
1493A
1g/$17.65
10g/$134.50
Soluble3
Green
405 nm
AEC
210.3 dal
3-Amino-9-ethyl Carbazole
A5754
10 g/$8.15
1372A
10 g/$26.10
Insoluble
Red
o-Dianisidine (free base)
D 9143
5 g/ $9.10
25 g/ $31.70
2060D
5 g/$8.45
25 g/$28.90
Soluble
Yellow-Orange
460 nm
o-Dianisidine´2 HCl (H2O soluble)
D 3252
p. 370
1 g/$7.95
5 g/$20.55
25 g/ $70.60
Soluble
Yellow-Orange
460 nm
4C1N
178.6 dal
4-Chloro-1-Naphthol
C 8890
p. 271
5 g/$21.30
1370C
1 g/$6.40
5 g/$21.80
10 g/$37.30
Insoluble
Blue
Guaiacol
2-Methoxyphenol
G 5502
100 g/ $14.60
Pyrogallol
1,2,3-Trihydroxybenzene
P 2923
25 g/ $19.80
420 nm4

References:
1. Sigma Chemical
2. Research Organics
3. ABTS Assay of Peroxidase
4. Pyrogallol Assay of Peroxidase
5. Purification procedure and extraction help from Websites



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  Last Modified on 7/15/2000

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