INTRODUCTORY BIOCHEMISTRYDNA Model
CHEMISTRY 330

Dr. Terry L. Helser, Professor of Chemistry
227 Physical Sciences Building, Oneonta, NY 13820-4015
Phone: (607)436-3518 or Email to: HELSERTL@oneonta.edu.

Study Guide - Biochemistry, The Molecular Basis of Life, 4th Ed., 2009, T. & J.R. McKee (Oxford Univ. Press)

Chapter 18.3: Regulation of Gene Expression

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How do we know that gene expression is regulated? List some ways.

History:

in 1961 F. Jacob and J. Monod proposed the ____________ model of _____________ operon regulation.
  1. What is an operon?
  2. What does it allow the cell to do?
  3. Do eukaryotes have them?
  4. What is the function of the inducer, repressor, operator, promoter and structural genes for the lactose operon?
  5. What is the inducer in this case?
  6. What are the functions of the Z, Y and Ac genes?
  7. Draw a diagram of this operon and correctly label the parts with the terms above.
The promoter sequence overlaps the operator sequence in the lac operon. Why is this essential to their functions?
What other regulatory system controls the lac operon?
  1. What is the catabolite that controls this system?
  2. What is the catabolite activator protein (CAP)?
  3. How does it control ß galactosidase (lactase) production?
What is the difference between positive and negative systems of gene regulation?
Fill in the following matrix of possible regulatory systems:

Regulation Type 

Control factor + 

Effector = 

Complex

Transcription Result 

Example 
+ 
?
?
Inactive 
Off
Unknown 

CAP 
Active 
Catabolite Repression


Lactose
On
? 
Repressor 
Active 
Repression 

In chapter questions: 18.17
End of chapter review questions: 21. Thought Questions: See above.

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  Last Modified on 7/16/2008

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