Introductory Biochemistry
CHEM 330-01 (CRN 470), CHEM 330-02 (CRN 913), CHEM 330-03 (CRN 914) & CHEM 330-04 (CRN 1420)
Class, Test Schedule (Room: HECO 132; M,W, F 9 AM) Fall, 2010
Lab Instructor: Dr. Terry L. Helser Office: PS 227 Phone: 607-436-3518 
E-mail To:
Text: Biochemistry, 4th Ed., 2008, T. & J. McKee (Oxford Publishers) 

Catalog Description: A survey of the basic biochemistry one needs to understand recent medical and biotechnological advances. The structure and functions of molecules and macromolecules in energy and genetic metabolism are studied. (LA Z) Prerequisite: CHEM 226 or 221.

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Class Schedule
Month & Days    Study Topic  
Reading Assignment
  I. Life's Structure Autobiography
August 25,27
Introduction, Co-op Class
Ch. 1
30, September 1,3
Cells, Atoms & Bonds
Ch. 2
pH & Water
Ch. 3
Amino Acids (Model Building)
Ch. 5
Protein Structure
Exam I (Friday, HECO 132, 9 AM) 
Ch. 4,6

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Month & Days
II. Energy Metabolism
Reading Assignment 
Sept. 29, Oct. 1
Fall Break - No Classes, Labs
27, October 4 
Enzymes & Energy
Ch. 6
6, 8 
Enzyme Kinetics
Ch. 6
11, 13, 15
Structure, Metabolism of Sugars

Ch. 7. Ch. 8

18, 20, 22

Krebs Cycle, Electron Transport
Ch. 9
25, 27 
Ox. Phosphorylation
Ch. 10
October 29 
Exam II (Friday, HECO 132, 9 AM)

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Month & Days
III. Genetic Metabolism
Reading Assignment 
November 1, 3, 5
Lipid Structure, Oxidation
Ch. 12
8, 10, 12
Nucleic Acids 
Ch. 17
15, 17, 19
Ch. 18.1, 18.2
Thanksgiving Vacation - No Classes
 29, December 1, 3
Transcription & The Genetic Code
Ch. 19
6, 8, 10
Translation & Control of Gene Expression
Ch. 18.3
December 15
Final Exam (Wednesday, room HECO 132, 
8 - 10:30 AM)

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Hourly Exams
2 x 100 pts. = 200 pts.
Scale: 90-100% A
Final Exam
200 pts.
80-89% B
Lab Research Paper
100 pts.
70-79% C
Laboratory Evaluations
200 pts.
60-69% D
Total: 700 pts.
0-59% E

You may earn up to 35 points for extra credit problem sets given throughout the semester. Tests have 10% more points than listed so I can challenge the best.

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Laboratory Schedule (Room: PSci 205 - Tue/Thr 9-1150 AM; Thr/Fri 1-4 pm) Fall, 2010
Required Materials: Graph Ruled Lab Notebook, Safety Goggles

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Month & Days
Laboratory Topic
Evaluation (Due Date)
 August 26, 27
No Labs
31, September 2, 3
Form Groups, Check In, Plan Buffer 
1-Oral Progress Rpt.
1-Prop (draft 9/3,7 or 8)
7, 9, 10

#1 Buffer Experiments 

1-Proposal (9/10, 14 or 15)
14, 16, 17
Buffer Experiments
21, 23, 24
Buffer Reports
#2 Enzyme Catalyzed Reactions
1- Oral Rpt. , Peer Eval.
2-Oral Progress Rpt.

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Month & Days
Laboratory Topic
Evaluation (Due Date)
28, 30, October 1
No Labs
1-Final Report (10/1)
2-Proposal (10/1, 5, 6)
5, 7 , 8
2-Proposal (10/8, 12, 13)
12, 14, 15
Enzyme Catalyzed Reactions 
2-Proposal (10/15, 19, 20)
19, 21, 22
Enzyme Reactions
#3 Enzyme Purification 
2-Oral Rpt. , Peer Eval. 
3-Oral Progress Rpt.
26, 28, 29
Enzyme Purification 
2-Final Rpt. (10/22, 26, 27) 
3-Proposal (10/22, 26, 27) 
November 2, 4, 5
Enzyme Purification 
3-Proposal (10/29, 11/2, 3)

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Month & Days
Laboratory Topic
Evaluation (Due Date)
9, 11, 12
Enzyme Purification
#4Cholesterol Analysis 
3-Oral Rpt. , Peer Eval.
3-Final Rpt. (11/12, 16, 17)
16, 18, 19
Cholesterol Analysis 
4-Proposal (11/12, 16, 17)
23, 25, 26
No Labs - Thanksgiving Break 
30, December 2, 3
Cholesterol Analysis 
 4-Proposal (11/19, 30, 12/1)
7, 9, 10
Cholesterol Analysis Report
The Gene Game (Check Out)
4- Oral Rpt. , Final Rpt. (12/10, 13)
December 15
Final Exam (Wednesday, room HECO 132, 
8-10:30 AM

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Lab Grading Policies:

Departmental policies apply to this course, except where modified or expanded below:

Your point total will be divided by the total possible for a % of 200 points. The final report for which you were primary author is worth a maximum of 100 points of your total grade.

Final reports must be typed, spaced ≥1.5 lines, and signed by all group members. They must contain an objective, introduction, procedure (flow diagram?), safety notes, results (an essay w/ tables, plots), discussion/conclusion, and reference sections.

The weekly proposals, with objective, equipment and reagents needed, procedure (flow diagram), safety notes and references, which proves you are prepared to do each experiment safely, must be accepted by your instructor before you will be allowed to do the lab ( submit an email no later than two days before the lab). One point /day will be subtracted for reports submitted after due dates.

Oral reports are awarded ≤100 points, of which 80% is for the group's presentation and 20% for the individual's stage presence, poise and participation. Generally, the score is an average of the peer evaluations from other teams and the instructor's. Copies of the printed grading form are available in the laboratory.
Lab notebooks may be collected at the instructor's whim.
Please note: You are to use your notebook to prepare for each lab with suitable notes on procedures, flow charts, tables for data, etc.; to make notes and take data during the lab; and to do calculations and write your results and conclusions after the lab. Each of these parts may be evaluated.

You must be credited as the major author of at least 1 lab report and score 60% on lab evaluations to pass the course. If you miss more than 1/3 of the labs, you will earn an E for the course, no matter what your test scores total. Plagiarism (see your Handbook ) will not be tolerated.
Approved clothing and safety goggles must be worn when any experimental work is occurring. Doing unauthorized experiments or procedures is prohibited. If in doubt, ask the instructor. All accidents involving personal injury, however minor, must be reported immediately to the instructor. The instructor has the right to expel anyone from the laboratory without credit for unsafe or offensive behavior or dress. 

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Introductory Biochemistry, Chemistry 330 Lab Information

1st Week, T/Th/F lab: 2nd Week: 4th Week:
Week after experiments are completed:
Oral (group) report ≤ 100 points
Group's proposal; Emailed by Fri,T or W ≤ 10 points
Final reports ≤ 100 points
Notebook, Quiz ≤ 10 points each
    - 1 point/day late off project total = % of 200 points total
    Final reports earn up to 100 points for the primary author out of the 300 point total for the lab part of the course. S/he must be clearly identified on the first page, and each member must be the primary author on at least one final report. A separate grade may be given for the primary author's work.

If you must miss a class, lab or group meeting, you must inform your group and instructor. Your group should decide on how you can repay your responsibilities to the group. Options might be to do extra library research, proposal or report writing, computer searches or whatever the group decides is adequate repayment for the loss of your participation. If the group decides your excuse was not valid, they may consider a loss of credit as appropriate punishment, and should so inform you and the instructor.

Team members who fail to participate adequately with their team may be fired, and justification must be provided to them and the instructor. The member must then find a new team to hire him/her or do the projects on their own, including all reports and proposals.

Penalties, generally -1 point a day for group reports which are late, will be assessed against the primary author of that report, not the rest of the group members, if requested by the group in writing or by email. 
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  Last Modified on 9/1/2010

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